2007 Projects
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Item Open Access Phylogeny of the Celastreae (Celastraceae) inferred using chloroplast and nuclear loci(Colorado State University. Libraries, 2007) Cappa, Jennifer J., author; Daw, Allison L., author; Simmons, Mark P., authorThe phylogenetic inference presented of the Celastreae (Celastraceae) is based on four loci: matK and trnL-F from the chloroplast genome and ITS and 26S rDNA from the nuclear genome. The species sampled are combined with taxa sampled from previous studies to better test the monophyly of the genera along with the intergeneric relationships within this tribe. The main purpose is to give an overview of the laboratory procedures used and the results from the sampling. The procedures include DNA isolation, DNA amplification, DNA purification, construction of contiguous sequences, sequence alignment, and lastly phylogenetic inference.Item Open Access Human-wildlife interactions and the Potential for Conflict Index(Colorado State University. Libraries, 2007) Hummer, Caroline, author; Vaske, Jerry J., author; Needham, Mark D., authorDetermine acceptability of management actions in scenarios varying by severity of human-wildlife interaction and species involved, using the Potential for Conflict Index and Analysis of VarianceItem Open Access Drawings from the Golden Age transformed(Colorado State University. Libraries, 2007) Thrasher, Rebekah L., authorThe purpose of this project was to find historical illustrations from the Gold Age of Fashion (approximately 1912-1930) and transform them into illustrations appropriate for the modern viewer. The original illustrations were updated from what some may consider a stagnant, two-dimensional piece of art into a creative interpretation of that picture, emphasizing fabric detail, figure movement, and overall mood.Item Open Access Effect of histone H3 E73D mutation on in vitro chromatin silencing(Colorado State University. Libraries, 2007) Colbert, Karen, author; Subramanian, Vidya, author; Thompson, Jeffrey, author; Luger, Karolin, authorOrganization of DNA into chromatin requires the presence of small basic proteins called histones. Core histones are highly conserved across various species. In addition to their function as DNA packing material, histones play an important role in the regulation of transcription, replication, and gene silencing. In particular, histone-mediated silencing is achieved via histone modifications and through interactions with suppressor proteins. In the yeast Saccharomyces cerevisiae, a single amino acid substitution (E73D) found within the conserved region of histone H3, has been shown to de-repress silencing at the telomeres and mating type loci in yeast (Thompson et al., 2003). It has been proposed that the substitution may shorten the amino acid side chain length enough to disrupt a necessary interaction at the H3-H4 interface within the nucleosome. Alternatively, the E73D mutation may affect the binding affinity of Sir3 for histone H3. Sir3 and Sir4, chromatin-associated repressor proteins, are known to mediate telomeric and mating loci silencing in S. cerevisiae (Grunstein et al., 1997). In order to test the effect of the E73D mutation on nucleosome stability in vitro, the crystal structure of nucleosomes containing the H3 mutant will be determined to ascertain the charge interactions between the amino acid residues within the vicinity of the substituted residue. In addition, binding studies will be carried out to investigate Sir protein binding to nucleosomes containing H3 E73D histones. Finally, analytical ultracentrifugation experiments will provide quantitative data on the in vitro dynamics of nucleosomal arrays comprised of nucleosomes containing H3 E73D.