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Characterization of Alstroemeria species and cultivars using random amplified polymorphic DNA (RAPD) analysis

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dc.contributor.authorPicton, Deric D., author
dc.contributor.authorHughes, Harrison G., advisor
dc.contributor.authorWard, Sarah, committee member
dc.contributor.authorStushnoff, Cecil, committee member
dc.date.accessioned2023-01-27T14:52:51Z
dc.date.available2023-01-27T14:52:51Z
dc.date.issued1997
dc.descriptionCovers not scanned.
dc.description.abstractThe characterization of Alstroemeria has not been effective through the use of conventional morphological markers. This is mainly due to the confused status of many of the species. Breeding of Alstroemeria has involved interspecific crosses as well as the use of mutagens and chromosome doubling for the creation of cultivars. Through the use of molecular techniques, in particular random amplified polymorphic DNA (RAPD) analysis, it is possible to uniquely characterize the species and cultivars. Fifteen species and hybrids along with 25 cultivars were examined using RAPD analysis. Four primers were eventually used for the analysis. All amplification products were separated using a 5% polyacrylamide gel and stained with silver nitrate for maximum differentiation of the fragments. The four primers yielded 73 amplification products which were polymorphic. When analysed using cluster analysis, all species and cultivars were uniquely characterized and putative parentages of many of the cultivars were determined.
dc.format.mediummasters theses
dc.identifier.urihttps://hdl.handle.net/10217/236153
dc.languageEnglish
dc.language.isoeng
dc.publisherColorado State University. Libraries
dc.relationCatalog record number (MMS ID): 991003472009703361
dc.relationQK495.A484P43 1997
dc.relation.ispartof1980-1999
dc.rightsCopyright and other restrictions may apply. User is responsible for compliance with all applicable laws. For information about copyright law, please see https://libguides.colostate.edu/copyright.
dc.subject.lcshLilies -- Analysis
dc.subject.lcshGenetic polymorphisms
dc.subject.lcshDNA fingerprinting of plants
dc.titleCharacterization of Alstroemeria species and cultivars using random amplified polymorphic DNA (RAPD) analysis
dc.typeText
dc.typeStillImage
dcterms.rights.dplaThis Item is protected by copyright and/or related rights (https://rightsstatements.org/vocab/InC/1.0/). You are free to use this Item in any way that is permitted by the copyright and related rights legislation that applies to your use. For other uses you need to obtain permission from the rights-holder(s).
thesis.degree.disciplineHorticulture & Landscape Architecture
thesis.degree.grantorColorado State University
thesis.degree.levelDoctoral
thesis.degree.nameDoctor of Philosophy (Ph.D.)

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